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Department of Biotechnology and Biochemistry

Department of Biotechnology was established in 2008, as a brand new department at Junagadh Agricultural University, with the mission of defining and establishing a new discipline; fusing life sciences with agriculture. The aim of this discipline is to advance fundamental understanding of how biological systems operate and to develop effective biology-based technologies for applications across a wide spectrum of agricultural needs through research and education. To offer innovative educational programmes with emphasis on integrating life sciences with a quantitative, systems-oriented engineering analysis and multidisciplinary approach at the undergraduate level and at the post graduate level for the M.Sc. and Ph.D.
Goals
1.   Genetic manipulation of crops for stress resistance.
2.   Molecular level interventions in functional genes for quality improvement.
3.   Modification of metabolic pathways for useful secondary metabolites.
4.   GMO’s for higher yields and for important phytochemicals, bio-diesels & natural pigments and microbes.
Objectives
1.   Teaching : Post graduate degrees of M.Sc. and Ph.D. in Molecular Biology and Biotechnology.
2.   Research : Plant Biotechnology.
3.   Develop infrastructural facilities to conduct routine post graduate teaching and research programmes.
4.   Establish analytical laboratory to cater the needs   of Biotechnological analysis of the research projects.
5.   Offer training to P.G. Students and research staff on analytical techniques in Biotechnological research.
6.   Conduct basic and strategic research to enhance production, productivity and quality of field crops.
7.   Develop facilities for molecular biology and genetic engineering work in plant sciences.
8.   Establish inter disciplinary research programmes with   Plant breeding, horticulture, Agronomy, Plant protection and post-harvest technology disciplines.
Professor and Head :
 dr_bagolakiya
Name: Dr. B. A. Golakiya
Address: Department of Biotechnology
Phone: 09979024255
E-mailThis email address is being protected from spambots. You need JavaScript enabled to view it.
Faculty :
Sr.
Name
Designation
Specialization
1
Dr. M. K. Mandavia
Professor
Biochemistry
2
Dr. S. V. Patel
Professor
Biochemistry
3
Dr. H. P. Gajera
Associate Professor
Biochemistry
4
Dr. Rukam S. Tomar
Associate Professor
Plant Breeding & Genetics
5
Dr. U. K. Kandoliya
Associate Professor
Biochemistry
6
Mr. Manoj V. Parakhia
Assistant Professor
Microbiology
7
Dr. P. J. Rathod
Assistant Professor
Biochemistry
8
Dr. Shradha Bhatt
Assistant Professor
Microbiology
9
Dr. Ashish G. Vala
Assistant Professor
Biotechnology
10
Ms. Viraj B. Rathod
Assistant Professor
Plant Breeding & Genetics
Office Staff
Sr.
Name
Designation
1
Mr. N. A. Patel
Agriculture Officer
2
Mr. Ashish Japda
Agriculture officer
3
Mr. V. R. Mori
Agriculture Assistant
4
Mr. S. H. Patel
Agriculture Assistant
5
Miss Sweta V. Bharadava
Agriculture Assistant
6
Mr. V. G. Vyas
Lab. Technician
7
Mr. R. L. Bhalara
Lab. Technician
8
Mr. V. K. Bhajaniya
Lab. Technician
9
Mr. H. Z. Hirpara
Lab. Technician
10
Miss. J. K. Manvar
Lab. Technician
11
Miss. N. S. Vaghani
Lab. Technician
12
Mr. S. I. Vindhani
Lab. Technician
13
Mr. D. M. Parmar
Senior Clerk
14
Mr. K. H. Khasdar
Pollinator
15
Mrs. G. H. Sardhara
Peon
Infrastructure facility :
Department of Biotechnology is having basic infrastructural research facilities for carrying out research on various aspects of Agricultural Biotechnology. There is a separate two storied building with 8 Research Labs., 15 offices of single span, two UG Labs., two lecture rooms and a Store. Various laboratories include; separation cell, biotech cell, microbial cell, msms cell, elemental cell, gfqc cell and training cell.
Equipment facility 
Click here to view Equipment facility
Facility for Genomics Study
Whole Genome Sequencer
(SOLiD 5500)
Genome Sequencer
(Ion Torrent)
Capillary Sequence
(16 Capillary)
Micromanipulator
Capillary Electrophoresis
RT PCR
Ultra Centrifuge
Multimode Reader
Gel Doc
Pico Drop
Liquid Handling System
Plate Centrifuge
Thermo cycler
Hydroshear
Blotting Device
Facility for Proteiomics Study
Surface Plasmon
Resonance (SPR)
2 D HPLC
Amino Acid Analyzer
HPLC
2 D Gel Electrophoresis with DIGE System
SNAP id
Protein Scanner 
Spot Picker and Digester
Solvent Extractor
NIR
Glutein analyser
Auto Titrando
Facility for Metabolomics  Study
LCMS
 
GCMS
Facility for Microbial Study
Scanning Electron Microscope
Flow Cytometer
Micro-manipulator
Photo Bioreactor
laminar flow
Fluorescence microscope
Media preparator
lyophilizer
BOD Analyser
Facility for Ionomics Study
ICPMS
XRF
FTIR
CHNS Analyzer
Micro Kjeldahl unit
VA Analyzer
General Equipments
KF Colimeter
Soxtherm
Sonicator
Centrifuge
Electrophoresis unit
Water bath
Analytical balance
pH meter
Translluminator
Hot plate
Vortex mixture
Microwave oven
Hot air oven
Incubator
Shaker
Moisture meter
Cyclone Grinder Mill
Water Purification System
Rancimat
UV-Spectrophotometer
Spectrofluorophotometer
Microtrac Particle analyzer
Rheometer
Fermenter
Automatic Autoclave
Microbalance
Digital Flame Photometer
Ice making machine
Deep freezer
Anaerobic jar
Research Priorities :
1.   Productivity Enhancement: Generation and utilization of male sterility systems for heterosis exploitation.
2.   Management of biotic stress: Identification of genes from different crops responsible of biotic resistance.
3.   Management of abiotic stress: Since Gujarat has the largest sea coast as well as desert area, management of abiotic stress becomes more important. So, understanding the molecular basis of tolerance to drought, high temperature and salinity; gene isolation, characterization and utilization for transgenic development in groundnut, cotton and vegetables.
4.   Molecular plant breeding: Gene mapping and marker assisted selection for productivity related traits and for biotic and abiotic stresses.
5.   Genomics and bioinformatics: Genome sequencing in Til, Groundnut, Castor and mango; functional genomics of abiotic stress tolerance in major crops of Gujarat; design and use of bioinformatics tool for use in structural, functional and comparative genomics, transcriptomics, proteomics and metabolomics.
6.   Development of transgenic: Transgenic for biotic and abiotic stress in different crops of Gujarat.
7.   Linkage maps: Development of saturated linkage maps.
8.   Gene Pyramiding: Gene Pyramiding major disease resistance genes in elite bajara and castor cultivars.
Major Achievements :
1.   Heat Sensitivity in wheat: The efflux of potassium during imbibition was lower in seeds from heat tolerant varieties compared to heat sensitive varieties. Studies using forty two varieties differing in heat sensitivity during early growth period based on yield under early shown conditions did not show any correlation between yield and efflux of any organic/inorganic solutes studied.
2.   Magnetism and plant productivity: Exposure of groundnut seeds to low magnetic field (100 gauss) enhanced germination and seed vigour index. 15-25% increase in pod yield was obtained in plants grown in pots, although kernel oil content was unaffected.
3.   Drought in groundunt: Drought at critical stages of plant life led to significant reduction in kernel oil content and oil yield in groundnut. Drought during pod development in groundnut plant, led to insignificant changes in protein, fall in lipids and increase in carbohydrates there by indicating that drought stress led to reduced conversion of carbohydrates to lipids. Differences in response of groundnut plants to drought under field and pot conditions indicated that pot experiments are best suited to characterize differences between varieties tolerant and sensitive to drought. Leaf total carbohydrates and total soluble sugars increased in response to drought and showed a negative correlation with pod yield. As the increase was greater in variety tolerant to drought these parameters may be useful as markers to screen for drought tolerance.
4.   Biochemical index of crop maturity: Along with seed/hull ratio in groundnut the content of free arginine (an amino acid) in the pods could be used as an index of pod maturity to fix harvest schedule in advances.
5.   Host pathogen interaction in Chickpea wilt: Total phenol content of root exudates was consistently higher in chickpea varieties resistant to fusarial wilt than in varieties tolerant to wilt. Phenolic compounds were effective inhibitors of fungal spore germination and mycelial growth and they inhibited the production and activity of fungal cell wall degredating enzymes involved in pathogenesis.Among the phenolic compounds, Chlorogenic acid, Salicylic acid and Hydroquinone were higher in tissues of resistant plants. Salicylic acid and Hydroquinone applied as soil drenching and foliar reduced the incidence of fusarial wilt of chickpea plants in field experiments.
6.   Crop quality in cropping systems: Quality parameters did not significantly change in groundnut, wheat and jowar in response to fertilizer inputs in a three year study in a permanent plot experiment.
7.   Salinity in wheat: Isoenzyme banding pattern of amylase was similar in response to salinity (up to 150 mM NaCl) in varieties known to differ in sensitivity to sodium chloride salinity.
8.   Host pathogen interaction in cumin wilt: Phenolic compounds were higher in root exudates of wilt tolerant cumin varieties. In root, stem and leaf tissues of tolerant cumin varieties at pre-infectional stage the concentrations of salicylic acid, hydroquinone and umbelliferone were higher than those in wilt susceptible varieties. Foliar application of former two phenolics in field experiments reduced the influence of wilt in cumin.
9.   Polyamines in drought response to groundnut: Water deficit stress (induced by PEG treatment) led to increase in the levels of total polyamines and individual polyamines in 15 day old seedlings.
10.  Biochemistry of fruit development in mango: Chemical, biochemical and metabolic indicators of fruit maturity in developing mango fruits indicate possibility of using selected indices for determining harvest times for quality.
Research schemes  :
Sr. No.
Name of the scheme/projects
Budget Head
1.
Research in Biotechnology at the Department of Biochemistry
12044-1
2.
Establishment of  Department of Biotechnology              
12401
3.
Improvement of Agricultural Production through Nanotechnological Interventions   
12402
4.
Molecular Mapping of Important Traits and their transfer through Marker Assisted Selection (MAS) in Groundnut and Cotton.
12029
5.
Food testing laboratory                   
18020
Externally Funded Project
Sr. No.
Project Title
Total Fund
Funding Agency
PI
1
Biochemical and Molecular Characterization of Bacillus spp. Isolated from Rhizosphere of Plants and Their Biocontrol Potentials against Fusarium oxysporum f. sp. cumini
14,49,420/-
Gujarat State Biotechnology Mission (GSBTM), DST, Govt. of Gujarat, Gandhinagar
Dr. H. P. Gajera
2
Synthesis and characterization of chitosan based NPK nanofertilizers
15,93,000/-
GSFC Science Foundation, Vigyan Bhavan, Vadodara - 391 750
Dr. H. P. Gajera
3
Genome and transcriptome sequencing of cumin to reveal insight of its genomic architecture.
53.94 Lakhs
Indian Council of Agricultural Research (ICAR)
Dr. Rukam S. Tomar
4
Transcriptome and Proteomic characterization for identification of candidate gene responsible for pistillate inflorescence and its reversion in castor.
65.00 Lakhs
Indian Council of Agricultural Research (ICAR)
Dr. Rukam S. Tomar
5
Transcriptome analysis in coriander for identification of candidate genes against stem gall disease.
51.73 Lakhs
Indian Council of Agricultural Research (ICAR)
Dr. Rukam S. Tomar
6
Genome and transcriptome sequencing of coriander to reveal insight of its genomic architecture and breeding targets.
25.41 Lakhs
Gujarat State Biotechnology Mission (GSBTM)
Dr. Rukam S. Tomar
7
QTL mapping and development of SCAR marker for Fusarium wilt and Macrophomina root rot in castor.
19.41 Lakhs
Gujarat State Biotechnology Mission (GSBTM)
Dr. Rukam S. Tomar
8
Proliferation of BT gene in native cotton verities of Gujarat.
20.02 Lakhs
Gujarat State Biotechnology Mission (GSBTM)
Dr. Rukam S. Tomar
9
Mapping of marine fish biodiversity using mtDNA barcoding.
19.68 Lakhs
Gujarat State Biotechnology Mission (GSBTM)
Dr. Rukam S. Tomar
10
Evaluation of fish meal substitution with plant proteins in formulated feed in rohu through nutrigenomics approach.
19.68 Lakhs
Gujarat State Biotechnology Mission (GSBTM)
Dr. Rukam S. Tomar
Training Organized
Sr. No.
Name of the Training
Sponsor
Duration/ Year
1
Students training on "Transgenic Trait Detection in Crop"
Gujarat State Biotechnology Mission, Gandhinagar
03 Days
(2018)
2
Winter School on “Genomic, Proteomic and Metabolomic Application in crop Improvement”
India Council of Agricultural Research (ICAR)
21 Days
(2017)
3
Teachers training on “Whole Genome Sequencing of Agriculturally Important Microbes”
Gujarat State Biotechnology Mission (GSBTM)
11 Days
(2015)
4
Teachers training on “Genomics, Proteomics and Bioinformatics tools for Plant Improvement”
Gujarat State Biotechnology Mission (GSBTM)
11 Days
(2013)
5
Winter School on “Instrumental Techniques in Agriculture and Food Assessment”
India Council of Agricultural Research (ICAR)
21 Days
(2011)
6
Student training on “Genomics, Transformation, Molecular marker and Bioinformatics tools for Plant Study and Improvement”
Gujarat State Biotechnology Mission (GSBTM)
11 Days
(2011)
Research Paper : NASS Rating Above 6
Sr. No
Research Paper Title
NAAS and Publisher
01
Rukam S Tomar, MA Iquebal, MV Parakhia, Deepak Kumar, Sarika Jaiswal, VM Rathod, SM Padhiyar, Neeraj Kumar, Anil Rai and Dinesh Kumar (2017). Draft whole genome sequence of groundnut stem rot fungus Athelia rolfsii revealing genetic architect of its pathogenicity and virulence. Nature Scientific Reports, Jul 13;7(1):5299. doi: 10.1038/s41598-017-05478-8.
11.23
02
Sarika Jaiswal, Tushar J. Antala, M. K. Mandavia, Meenu Chopra, Rahul Singh Jasrotia, Rukam S. Tomar, Jashminkumar Kheni, U. B. Angadi, M. A. Iquebal, B. A. Golakia, Anil Rai and Dinesh Kumar (2018). Transcriptomic signature of drought response in pearl millet (Pennisetum glaucum (L.) and development of web-genomic resources. Nature Scientific Reports, Feb 13; 8(1):3382. DOI:10.1038/s41598-018-21560-1
10.26
03
H. P. Gajera, Disha D. Savaliya, S. V. Patel, B. A. Golakiya (2015). Trichoderma viride induces pathogenesis related defense response against rot pathogen infection in groundnut (Arachis hypogaea L.). Infection, Genetics and Evolution, 34: 314–325 DOI :http://dx.doi.org/10.1016/j.meegid.2015.07.003
9.26
04
Samir A. Anuj, Harsukh P. Gajera, Darshna G. Hirpara, Baljibhai A. Golakiya (2018). Bactericidal assessment of nano-silver on emerging and re-emerging human pathogens. Journal of Trace Elements in Medicine and Biology, Online DOI:https://doi.org/10.1016/j.jtemb.2018.04.028
9.23
05
Rukam S. Tomar, M.V. Parakhia, V.M. Rathod, J.R Thakkar, S.M. Padhiyar,V.D. Thummar, H. Dalal, V.V. Kothari, JasminkumarKheni, R.M. Dhingani, Pritesh Sabara, B.A. Golakiya (2016). Molecular mapping and identification of QTLs responsible for charcoalrot resistance in Castor (Ricinus communisL.). Industrial Crops and Products 95, 184–190.
9.18
06
H. P. Gajera, Darshna G. Hirpara, Zinkal A. Katakpara, S. V. Patel, B. A. Golakiya (2016). Molecular evolution and phylogenetic analysis of biocontrol genes acquired from SCoT polymorphism of mycoparasitic Trichoderma koningii inhibiting phytopathogen Rhizoctonia solani Kuhn. Infection, Genetics and Evolution, 45:383–392. DOI:http://dx.doi.org/10.1016/j.meegid.2016.09.026
9.02
07
Shah, K. A.; Kulkarni, G.U.; Tomar, R. S. and Bhalala, M.K. (2007). Heterosis for yield and yield related traits in red chilli (Capsicum annuum var. Longum (DC) Sendt). Int. J. of Biosci. Rep., 5(1): 355-362.
8.91
08
Shah, K. A.; Kulkarni, G.U.; Tomar, R. S. and Bhalala, M.K. (2007). Combining ability studies for yield and yield related traits in red chilli (Capsicum annuum var. Longum (DC) Sendt). Int. J. of Biosci. Rep., 5(1): 345-354.
8.91
09
Nakum, V.H.; Rukam, S. Tomar; Kulkarni, G.U and Patel, H.B. (2007). Evalution of coconut (Cocos nucifera L.) cultivars for age of tender nuts in different season. Int. J. of Biosci. Rep.,5 (1): 79-81.
8.91
10
Patel, H.B.; Rukam, S. Tomar and Kulkarni, G.U. (2007). Effect of plant growth regulators on sex expression and yield of sponge gourd (luffa cylingrica R.) Cv. ‘PusaChikni’. Int. J. of Biosci. Rep., 5 (1): 21-24.
8.91
11
Patel, H.B.; Rukam, S. Tomar and Kulkarni, G.U. (2007). Economics of sponge gourd (luffa cylingrica R.) as influenced by plant growth regulators. Int. J. of Biosci. Rep., 5 (1): 53-55.
8.91
12
Parmar, J.K ; Patel, J. J. ; Tomar, R. S. and Gangwal, T.V. (2007). Effect of inorganic, organic and azotobacter on yield, protein and nutrient uptake by grain Amarauthus. Int. J. of Biosci. Rep., 5 (1): 195-199.
8.91
13
Rukam S. Tomar, S. N. Jaimini and Ran S. Tomar (2006). Genetic Variability, Correlation and Path Analysis in Neem (Azadirachtaindica A. Juss). Int. J. of Biosci. Rep., 4 (1): 49-53.
8.91
14
Rukam S. Tomar, S. N. Jaimini and Ran S. Tomar (2006). Variability in Neem (Azadirachtaindica A. Juss). Int. J. of Biosci. Rep., 4 (1): 107-110.
8.91
15
Rukam S. Tomar, Ramesh K. Solanki, S. N. Jaimini and Ran S. Tomar (2006). Floral and Reproductive Biology of Neem (Azadirachtaindica A. Juss). Int. J. of Biosci. Rep., 4 (1): 87-89.¬
8.91
16
Darshna G. Hirpara, H. P. Gajera* (2018) Molecular heterozygosity and genetic exploitations of Trichoderma inter-fusants enhancing tolerance to fungicides and mycoparasitism against Sclerotium rolfsii Sacc. Infection, Genetics and Evolution, 66: 26-36. DOI:https://doi.org/10.1016/j.meegid.2018.09.005
8.89
17
Darshna G. Hirpara, H. P. Gajera*, Jaydeep G. Hirapara, B. A. Golakiya (2017) Inhibition coefficient and molecular diversity of multi stress tolerant Trichoderma as potential biocontrol agent against Sclerotium rolfsii Sacc. Infection, Genetics and Evolution, 55: 75–92. DOI:http://dx.doi.org/10.1016/j.meegid.2017.08.029
8.89
18
Darshna G. Hirpara, H. P. Gajera, R. D. Bhimani, B. A. Golakiya (2016). The SRAP based molecular diversity related to antifungal and antioxidant bioactive constituents for biocontrol potentials of Trichoderma against Sclerotium rolfsii Scc. Current Genetics, 62:619–641. DOI 10.1007/s00294-016-0567-5
8.68
19
Rasmieh Hamid, Rukam S Tomar, Saeid Malekzadeh Shafaroudi, Balaji A Golakiya and Motahhareh Mohsenpour (2018). Transcriptome profiling and cataloguing differential gene expression in floral buds of fertile and sterile lines of cotton (Gossypium hirsutum L.). Gene, 660: 80–91. Doi:10.1016/j.gene.2018.03.070.
8.42
20
H.P.Gajera and D.N.Vakharia (2010). Molecular and biochemical characterization of Trichoderma isolates inhibiting a phytopathogenic fungi Aspergillus niger Van Tieghem. Physiological and Molecular Plant Pathology, 74: 274-282.
8.2
21
H. P. Gajera, R. P. Bambharolia, Darshna G. Hirpara, S. V. Patel, B. A. Golakiya (2015). Molecular identification and characterization of novel Hypocrea koningii associated with azo dyes decolorization and biodegradation of textile dye effluents. Process Safety and Environmental Protection, 98: 406–416. DOI: http://dx.doi.org/10.1016/j.psep.2015.10.005
7.83
22
H. P. Gajera, Zinkal A. Katakpara, S. V. Patel, B. A. Golakiya (2016). Antioxidant defense response induced by Trichoderma viride against Aspergillus niger Van Tieghem causing collar rot in groundnut (Arachis hypogaea L.). Microbial Pathogenesis, 91: 26-34. DOI: http://dx.doi.org/10.1016/j.micpath.2015.11.010p
7.79
23
Darshna G. Hirpara, Harsukh P. Gajera, Hitesh Z. Hirpara, Balubhai A. Golakiya (2017). Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists. Journal of Molecular Microbiology and Biotechnology, 27:22–28. DOI: 10.1159/000452997
7.70
24
M.K.Mandavia, N.A.Khan, H.P.Gajera, J.H.Andharia and M.Parameswaran (2000). Inhibitory effects of phenolic compounds on fungal metabolism in host pathogen interactions in fusarium wilt of cumin. Allelopathy Journal, 7 : 85-92.
7.5
25
H. P. Gajera, R. P. Bambharolia, Rinkal K. Domadiya, S. V. Patel and B. A. Golakiya (2014). Molecular characterization and genetic variability studies associated with fruit quality of indigenous mango (Mangifera indica L.) cultivars. Plant Systematics and Evolution, 300: 1011-1020. DOI 10.1007/s00606-013-0939-y
7.31
26
Manoj V. Parakhia , Rukam S. Tomar, Megha R. Vadukia, Bipin J. Malviya, Visha M. Rathod, Jalpa R. Thakkar, Kinjal J. Parmar, Rashmin M. Dhingani and B. A. Golakiya (2014). Draft Genome Sequence of the Methyl Parathion (Pesticide) Degrading Bacterium Pseudomonas spp. MR3. Indian Journal of Microbiology,54(1):120-121.
7.29
27
Manoj V. Parakhia , Rukam S. Tomar, Bipin J. Malviya, Rashmin M. Dhingani, Visha M. Rathod, Jalpa R. Thakkar and B. A. Golakiya (2014). Draft Genome Sequence of the Endophytic Bacterium Enterobacter spp. MR1, Isolated from Drought Tolerant Plant (Butea monosperma). Indian Journal of Microbiology, 54(1):118-119.
7.29
28
H. P. Gajera*, Shila N. Gevariya, S. V. Patel, B. A. Golakiya (2018) Nutritional profile and molecular fingerprints of indigenous black jamun (Syzygium cumini L.) landraces. Journal of Food Science and Technology, 55(2): 730-739. DOI:https://doi.org/10.1007/s13197-017-2984-y
7.26
29
H. P. Gajera*, Shila N. Gevariya, Darshna G. Hirpara, S. V. Patel, B. A. Golakiya (2017) Antidiabetic and antioxidant functionality associated with phenolic constituents from fruit parts of indigenous black jamun (Syzygium cumini L.) landraces. Journal of Food Science and Technology, 54(10):3180–3191. DOI:https://doi.org/10.1007/s13197-017-2756-8
7.24
30
H.P.Gajera, M.B.Kapopara and V.H.Patel (2010). Application of peanut butter to improve fatty acid composition of biscuits. Journal of Food Science and Technology, 47: 285-289.
7.2
31
H.P.Gajera, M.B.Kapopara, V.H.Patel and M.M.Patel (2008). Influence of peanut butter on quality characteristics of biscuits. Journal of Food Science and Technology, 45: 373–375.
7.2
32
H.P.Gajera and D.N.Vakharia (2012). Production of lytic enzymes by Trichoderma isolates during in vitro antagonism with Aspergillus niger, the causal agent of collar rot of peanut. Brazilian Journal of Microbiology, 43(1): 43-52. DOI-http://dx.doi.org/10.1590/S1517-83822012000100005-on line.
7.1
33
C.V. Patel, H.P. Gajera*, H.Z. Hirpara and B.A. Golakiya (2017). Induction of Antioxidant Enzymes and Isozymes Profile of Chickpea Genotypes Under Compatible and Incompatible Interactions with Fusarium Wilt. Journal of Plant Pathology, 99 (1):131-140. DOI:http://dx.doi.org/10.4454/jpp.v99i1.3854
7.04
34
H.P.Gajera, Khyati Rathod, S.V.Patel, and B.A.Golakiya (2013). Biochemical markers induced by Trichoderma against rot infection in groundnut (Arachis Hypogaea L.) and their appraisal with DNA fingerprinting. Canadian Journal of Plant Protection, 1(2): 49-63.
7.0
35
H. P. Gajera, R. S. Tomar, S. V. Patel, R. R. Viradia and B. A. Golakiya (2011). Comparison of RAPD and ISSR markers for genetic diversity analysis among different endangered Mangiferaindica genotypes of Indian Gir forest region. J. Plant Biochem. Biotechnol., 20(2):217–223.
6.95
36
H.P.Gajera, R.P.Bambharolia, S.V.Patel, T.J.Khatrani, B.A.Goalkiya (2012). Antagonism of Trichoderma spp. against Macrophomina phaseolina: Evaluation of Coiling and Cell Wall Degrading Enzymatic Activities. Journal of Plant Pathology and Microbiology, 3:149. DOI-http://dx.doi.org/10.4172/2157-7471.1000149 - open access
6.9
37
H.P.Gajera, R.S.Tomar, S.V.Patel, R.R.Viradia and B.A.Golakiya (2011). Comparison of RAPD and ISSR markers for genetic diversity analysis among different endangered Mangifera indica genotypes of Indian Gir forest region. Journal of Plant Biochemistry and Biotechnology, 20(2): 217-223.
6.8
38
H. P. Gajera, Disha D. Savaliya, S. V. Patel, B. A. Golakiya (2015). Lipoxygenase-related defense response induced by Trichoderma viride against Aspergillus niger Van Tieghem, inciting collar rot in groundnut (Arachis hypogaea L.). Phytoparasitica, 43: 229–240. DOI 10.1007/s12600-014-0435-0
6.72
39
H. P. Gajera, J. K. Jadav, S. V. Patel, B. A. Golakiya (2014). Trichoderma viride induces phenolics in groundnut (Arachis hypogaea L.) seedlings challenged with rot pathogen (Aspergillus niger Van Tieghem). Phytoparasitica, 42: 703-712. DOI 10.1007/s12600-014-0413-6
6.72
40
Darshna G. Hirpara, H. P. Gajera, H. Z. Hirpara, B. A. Golakiya (2017). Molecular diversity and fingerprints of Trichoderma associated with antagonistic potentials against Sclerotium rolfsii Sacc. Journal of Plant Diseases and Protection, 124:31–40. DOI 10.1007/s41348-016-0053-9
6.68
41
Kndoliya U. K. and D. N. Vakhariya (2013), Antagonistic effect of Pseudomonas fluorescens against fusrium oxysporum f.sp. Ciceri wilt in chickpea. Legume Research.,36(6) : 569 - 575.
6.5
42
Tomar Rukam S., Parakhia M. V., Thakkar J. R., Rathod V. M., Padhiyar S. M., Thummar V. D., Dalal H., Kothari V.V., Kheni J. V., Dhingani R. M. and Golakiya B. A. (2016). Development of linkage map and identification of QTLs responsible for fusarium wilt resistance in castor (Ricinuscommunis L.). Research Journal of Biotechnology, 11 (5): 67-73.
6.42
43
Yusufzai S. I., PadhiyarShital M., Lende Smit , Tomar Rukam S.,ThummarVibha D., Thakkar Jalpa R., RathodVisha M., Kheni Jasmin V. , Kothari Vishal , Parakhia M. V. and Golakiya B. A. (2016). Genetic Diversity Analysis in Some Marine Fish Species of Gujarat Coast through Morphological and Molecular Markers. Research Journal of Biotechnology, 11 (3): 77-84
6.42
44
Tomar Rukam S., Parakhia M. V., Rathod V. M., Kheni J. V., Thakkar J. R., Thummar V. D., Padhiyar S. M. and Golakiya B. A. (2015). Development of Inter Simple Sequence Repeat based SCAR marker for sex determination in Carica papaya. Research Journal of Biotechnology, 10 (12): 91-97.
6.42
45
Kapadia C. V., Mahatma M. K., Parekh M. J., Patel Nafisa and Tomar R. S. (2015). Identification of Resistance Gene Analogs (RGAS) from Highly Wilt Resistant Castor (RicinusCommunis L.) Genotype. Research Journal of Biotechnology, 10 (5): 16-26.
6.42
46
C. V. Patel, H. R. Ramani, H. P. Gajera (2017). Genotypic study of eighteen Chickpea (Cicer arietinum L.) against Fusarium oxysporum f.sp. ciceri with the help of STMS marker using Capillary electrophoresis and Seed storage protein profiling. Research Journal of Biotechnology, 12(7): 83-89
6.24
47
Tomar Rukam S., Parakhia M. V., Thakkar J. R., Rathod V. M. and Golakiya B. A. (2014). A Comparative analysis of ISSR and RAPD markers for studying genetic diversity in Trigonellafoenum-graecum genotypes. Research Journal of Biotechnology, 9 (10): 89-95.
6.23
48
Tomar Rukam S., Kulkarni G. U., Parakhia M. V., Thakkar J. R., Rathod V. M., Solanki R. K. and Golakiya B. A. (2014). Genetic diversity analysis in coriander (Coriandrumsativum) genotypes through morphological and molecular characterization. Research Journal of Biotechnology, 9 (3): 1-11.
6.23
49
Tomar Rukam S., Parakhia M. V., Kavani R. H., Dobariya K. L., Thakkar J. R., Rathod V. M., Dhingani R. M. and Golakiya B. A. (2014). Characterization of castor (Ricinuscommunis L.) genotypes using different markers. Research Journal of Biotechnology, 9 (2): 6-13.
6.23
50
Rukam S. Tomar, H. P. Gajera, R. R. Viradia, S. V. Patel and B. A. Golakiya (2014). Characterization of mango genotypes of Gir region based on ISSR markers. Indian Journal of Horticulture, 71(1): 1-5.
6.15
Books published: 
Sr. 
Title of books
 
Authors
1
Biochemistry at a glance
:
D.N. Vakharia, S.V. Patel, U.K. Kandoliya and J.J. Dhruve
2
A Glossary of Biochemistry and Biotechnology
:
S.V. Patel, U.K. Kandoliya, M.K. Mandavia, J.J. Dhruve, D.N. Vakharia, B.A. Golakiya and S. G. Savaliya
3
Fundamentals of Biochemistry, A Text Book
:
H.P. Gajera, S.V. Patel, B.A. Golakiya
4
Handbook of Genetics and Biotechnology.
:
Rukam S. Tomar, Sunil V. Patel, H. P. Gajera and B. A. Golakiya
5
Molecular Biology and Biotechnology
:
Manoj V. Parakia, Rukam S. Tomar, Sunil Patel and B. A. Golakiya
6
Molecular markers and Plant Biotechnology
:
Rukam S. Tomar, Manoj V. Parakia,  Sunil Patel and B. A. Golakiya
7
Hand Book of Life Science
:
Sunil Patel, Rukam S. Tomar, Harsukh Gajera, B.A. Golakiya and Manoj V Parakhia
8
DNA- A Bridge Between Biochemistry and Biotechnology
:
J.J. Dhruve, U.K. Kandoliya, Sunil V. Patel and B.A. Golakiya
9
Illustrated Dictionary of Microbiology
:
S.V. Patel, B.A. Golakiya, L. F. Akbari and Valentina V. Umrania
10
Radio Tracer Techniques for Agriculturists and Biologists
:
B.A. Golakiya, H. L Sakarvadia, Poonam I. Jetpara, K. B. Parmar and S.V. Patel
11
Glimpse of Plant Physiology
:
Chetana Mandaviya, S. V. Patel, M. K. Mandaviya, B. A. Golakiya and V. P. Chovatia
12
Glossary of Soil Sciences
:
S.V. Patel, H.P. Gajera, S. G. Savalia and B.A. Golakiya
13
Text Book of Soil Physics
:
S. G. Savalia,  B.A. Golakiya  and S.V. Patel
Accomplishments :
Sr. No.
Items
Number
1
No. of Research paper published                                    
68
2
Books
12
3
Popular articles
30
4
Laboratory manuals
03
5
Radio talk
01
6
Number of ongoing research schemes
02
7
Number of Project completed
31
8
Number of internal ongoing projects
06
9
Number of internal project proposed
03
10
Number of externally funded project
01
11
Number of Training attended by staff
15
12
Number of Seminar/workshop attended by staff
05
13
National Seminar organized
01
14
Workshop organized
03
15
Training/winter school organized
06
16
Number of students trained
62
17
Number of students with Dissertation
64
18
Number of students passed out
22
19
Sequence Registration to NCBI
03
20
Number of Recommendations for farmers
05
21
Number of Recommendations for scientific community
02
Teaching Programmes : 
Biochemistry:
No. of Students Passed in M Sc (Agri) : 42
No. of Students Passed in Ph D : 18
No. of Students Currently enrolled in M Sc (Agri) : 08
No. of Students Currently enrolled in Ph D : 01
Biotechnology: 
No. of Students Passed in M Sc (Agri) : 101
No. of Students Passed in Ph D : 32
No. of Students Currently enrolled in M Sc (Agri) : 09
No. of Students Currently enrolled in Ph D : 05
Total : SRF / JRF / NET : 39
Future Thrust :
1.   DNA fingerprinting of important crops of Gujarat.
2.   DNA fingerprinting of important horticultural and forest  trees.
3.   Development of markers for marker assisted selection.
4.   Molecular mapping and tagging of stress tolerant genes /QTLs in  different crops.
5.   Isolation and molecular characterization of bacteria to develop efficient biofertilizers.
6.   Antiviral and antimicrobial compounds in plant : Collection and characterization.
7.   Characterization of microorganism present in the soil and water through metagenomics.
8.   Factor affecting nutritional quality traits in food crops important for national/International market.
9.   Strengthening of Department of Biotechnology as centre of research in proteomics and bionanotechnology.
FOOD TESTING LABORATORY (NABL Accredited)
Objective:
1.   To provide professional service of Quality Control & QA
2.   To help promote Agri-product export through analytical balkne
3.   To Provide analytical support to agro processing industries
4.   To Provide analytical support to agro-processing industries. 
DNA LEARNING CENTRE
Objective:
1.   Vocational training
2.   Regular Training
3.   Project work/Dissertation for PG student
4.   Training to professionals & Government nominees
Course: Whole Genome Sequencing, DNA fragment sequencing, DNA isolation, purification, quantification, RAPD, RFLP, AFLP, SNP analysis, SSCP Analysis, SPA, Si RNA, St.RNA Design and Synthesis, cloning-sequencing up stream & down stream, disease diagnosis, clinical biotech analysis, etc.
Duration: 2 days, One week, two week, three week, six week, eighteen week
Training Fees: Available on Reques